Knowledge-enriched characterization of mobile populations. Morphological facts from photos can incorporate for the richness of flow cytometry facts. As an example, the figure demonstrates an normally standard apoptosis assay using Annexin V and PI, including mobile imaging to characterize cells in each population to reveal morphologically distinctive features. These insights could not are received from multiplex staining on your own.
As samples are acquired on the Attune CytPix Flow Cytometer, the substantial-speed brightfield digicam captures and retailers images of detected situations at a amount approximately six,000 illustrations or photos for every 2nd, according to the flow rate and picture measurement. For bigger flexibility, Attune Cytometric Computer software lets you regulate the impression capture frequency as required and choose a particular gate for image capture, if required.
Combine acoustic concentrating technology with high-parameter Examination for versatile spectral unmixing and conventional workflows.
Learn new insights into your experimental data by visualizing populations applying blended fluorescence and morphology details. Our software interface wizards allow downsampling, concatenation (merged documents) and dimensionality resources which might be crafted into your software for an intuitive, streamlined knowledge analysis working experience.
"An image isn't pretty truly worth one thousand dots, nevertheless it truly really helps to see images. It is great to see photographs that correlate with staining. For instance, AnnexinV-constructive cells that happen to be morphologically distinct than healthful cells, or CD14+ monocytes which are larger and much more textured than CD3+/CD4+/CD14- lymphocytes.
Furthermore, it's illuminating to determine what number of doublets slip by means of traditional singlet gates, and terrific to verify the position of surprising double-good occasions in my Evaluation."
Optimizing gating tactic. Even sturdy manual singlet gating is error-vulnerable and remains a subjective conclusion position in Practically all flow cytometry assays. Imaging can be employed to substantiate and adjust gates to include only single cells of desire.
Regular image high quality even at high throughput Acoustic concentrating as well as a substantial-speed camera Incorporate to impression these T read more cells regularly at small or high flow rates. Very easily change concentrate and camera options to fulfill experimental needs.
The Attune NxT cytometer could be the trustworthy workhorse instrument your lab can count on for constant and dependable functionality. The Attune CytPix model includes a large-speed brightfield digital camera and AI-pushed image analysis program, with modern updates including machine Studying applications and an intuitive user interface, enabling enhanced info precision, precision, and quicker insights into sample biology.
Consumers can check out function illustrations or photos in serious-time to substantiate sample good quality, optimize protocols, and document the morphology of every mobile population discovered for Evaluation. Imaging capture also Positive aspects from acoustic concentrating, which helps you to place the cells to ensure that a pointy, centered impression is attained.
Seem waves enable exact cell alignment, causing exceptional facts good quality and ~10X speedier sample flow premiums.
We Formerly shown the power of imaging Motor vehicle-T/Ramos cell interactions. Permit’s examine just the inhabitants of best curiosity, the double positive functions, to learn more. We will now use extended morphological parameters (listed here, circularity and skewness of depth) to further more take a look at the capabilities of such populations and refine gating on these functions, increasing facts robustness.
Created-in dimensionality reduction resources increased resolution into populations. Fluorescently gated populations again-gated on to UMAP plot to substantiate expected inhabitants resolution in lysed entire human blood. User can see occasions from Every cluster for Visible confirmation of mobile phenotypes.
Adaptable payment prepare from Agilent Economical Methods enable you to get the important instrumentation you need for rapid task, or to develop your lab.
At the center of each and every Attune Flow Cytometer lies our groundbreaking acoustic concentrating technology, which makes use of sound waves to meticulously align cells in the flow mobile. This impressive tactic surpasses conventional hydrodynamic solutions, resulting in quicker processing speeds, Remarkable info excellent, and increased sample throughput.
Because the mobile line experienced Earlier been analyzed on the Attune CytPix Flow Cytometer, the scientists went back to the images and were in the position to doc the microbial infection at least five times earlier.
Agilent Benefit Promise assures you not less than a decade of instrument use in the date you buy. Otherwise, We are going to credit history you Together with the residual worth of the technique towards an upgraded product.
What gave the impression to be a homogeneous inhabitants is really much more varied—an insight that needs to be thought of when interpreting benefits.